Figure 8. Dorsal-to-ventral maturation of wolframin expression in the medial entorhinal cortex and parasubiculum.
(a) Tangential sections of the MEC and PaS (outlines dashed) double-stained for calbindin-immunoreactivity (green) and wolframin immunoreactivity (red) in a P4 rat. Shown is an overlay of red and green fluorescence. (b) as (a) for a P8 rat. (c) as (a) for a P20 rat. Wolframin is present in the dorsal ~10% of the parasubiculum at P4, ~40% at P8 and 100% at P20. Note that wolframin expression co-localizes with calbindin-expression in the MEC (hence the yellowish color) and increases from dorsal to ventral with age. Scale bars 250 µm. D- Dorsal; V- Ventral; M- Medial; L- Lateral. Orientation in (a) applies to all sections.