Table 3.

Effect of age and immobilization on TNNT3 splice forms.

TNNT3 splice forms	Relative Abundance
	2-month		9-month		18-month
	Control limb	Immobilized limb	Control limb	Immobilized limb	Control limb	Immobilized limb
α1	0.197 ± 0.033	0.114 ± 0.018*	0.240 ± 0.040	0.080 ± 0.008*	0.586 ± 0.026	0.274 ± 0.034*
α2	0.038 ± 0.005	0.031 ± 0.004	0.030 ± 0.007	0.012 ± 0.001	0.093 ± 0.006	0.054 ± 0.007*
α3	0.028 ± 0.004	0.025 ± 0.004	0.024 ± 0.003	0.009 ± 0.001*	0.048 ± 0.006	0.027 ± 0.004*
β1	0.015 ± 0.001	0.019 ± 0.001*	0.010 ± 0.001	0.013 ± 0.001	0.004 ± 0.001	0.006 ± 0.001*
β2	0.018 ± 0.002	0.017 ± 0.002	0.013 ± 0.003	0.006 ± 0.001	0.022 ± 0.004	0.015 ± 0.002
β3	0.322 ± 0.017	0.315 ± 0.013	0.340 ± 0.026	0.467 ± 0.019	0.162 ± 0.010	0.378 ± 0.028*
β4	0.029 ± 0.003	0.081 ± 0.009*	0.023 ± 0.006	0.074 ± 0.017*	0.010 ± 0.002	0.051 ± 0.007*
β5	0.054 ± 0.008	0.025 ± 0.002*	0.038 ± 0.005	0.017 ± 0.001*	0.005 ± 0.001	0.004 ± 0.001
β6	0.088 ± 0.007	0.108 ± 0.005*	0.062 ± 0.006	0.105 ± 0.004*	0.032 ± 0.004	0.092 ± 0.008*
β7	0.096 ± 0.006	0.099 ± 0.006	0.081 ± 0.015	0.097 ± 0.010	0.019 ± 0.002	0.052 ± 0.004*
β8	0.034 ± 0.006	0.054 ± 0.006*	0.023 ± 0.002	0.041 ± 0.006*	0.004 ± 0.001	0.013 ± 0.002*
β9	0.098 ± 0.011	0.110 ± 0.006	0.056 ± 0.008	0.077 ± 0.005*	0.015 ± 0.002	0.034 ± 0.003*
TNNT3 splice form	Two-way ANOVA (nested)
	Effect of Age		Effect of Immobilization		Age * Immobilization
	F statistic	p value	F statistic	p value	F statistic	p value
α1	65.1542	<.0001	52.5714	<.0001	10.7655	0.0003
α2	44.7273	<.0001	19.4619	0.0001	4.8226	0.0153
α3	9.9045	0.0005	13.0311	0.0011	2.4487	0.1035
β1	76.5313	<.0001	11.5078	0.002	0.1905	0.8275
β2	5.2331	0.0112	4.237	0.0483	0.7098	0.4998
β3	30.0513	<.0001	31.4339	<.0001	19.5423	<.0001
β4	6.8984	0.0034	61.1599	<.0001	0.3459	0.7104
β5	38.6427	<.0001	21.6376	<.0001	6.1588	0.0057
β6	19.8269	<.0001	60.6869	<.0001	5.9859	0.0065
β7	54.4016	<.0001	9.0167	0.0054	2.8995	0.0706
β8	38.1622	<.0001	17.4841	0.0002	0.9474	0.3991
β9	79.9899	<.0001	9.757	0.0039	0.2294	0.7964
Total TNNT3α	62.8523	<.0001	49.4036	<.0001	10.2252	0.0004
Total TNNT3β	65.0894	<.0001	51.161	<.0001	10.5915	0.0003
PC1	60.6813	<.0001	54.0919	<.0001	13.224	<.0001
PC2	33.7262	<.0001	0.9318	0.3421	8.3441	0.0013

The mean ± S.E.M relative abundance of the TNNT3 splice forms in gastrocnemius muscle from immobilized and contralateral non-immobilized (control) limbs of 2-, 9-, and 18-month old rats is presented. The first two principal components and the results from two-way nested ANOVA for age, immobilization and interaction (Age * Immobilization) effects are shown. Statistically significant changes (p<0.05) determined by paired Student’s t-test are indicated. n=6–7 per group.

^*compared to the control limb for that particular age group.