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. Author manuscript; available in PMC: 2016 Nov 24.
Published in final edited form as: ACS Nano. 2015 Oct 27;9(11):11325–11332. doi: 10.1021/acsnano.5b05055

Figure 2.

Figure 2

Mapping the latch sensing zone of the α-HL nanopore with blocks of G:C base pairs in a background of A:T base pairs. (A) The hairpin duplex sequences studied. (B) Representative structure for α-HL and the hairpin duplex based on pdb 7AHL,29 1JVE,38 4HW1.39 (C) Current blocking histograms for mixtures of analyte and standard duplexes. The hp-1 sequence was used as an internal standard, and it was always mixed with the analyte strand in a ratio of 1:2, respectively; therefore, the smaller peak area always represents hp-1. (D) Table of peak-to-peak ΔI/Io and current differences measured between the internal standard (hp-1) and the analyte strand. The error in each value represents the standard deviation of peak-to-peak widths from three individual protein channels. The data were recorded with 10 mM KPi (pH 7.4), 1.00 M KCl, at 22 ± 1 °C, and a 100 mV (trans vs. cis) bias. The histograms represent > 200 recorded events.