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. Author manuscript; available in PMC: 2016 May 23.
Published in final edited form as: J Immunol. 2010 Jun 2;185(1):23–27. doi: 10.4049/jimmunol.1000832

FIGURE 1.

FIGURE 1

Development of a GFP-inducible system to detect FCRL6 engagement. A 43-1 cells transduced with the FCRL6ζ reporter construct were stained with the anti-FCRL6 7B7 mAb (black line) or with an isotype-matched control (grey shade). B Untransduced 43-1 control (grey columns) or 43-1 FCRL6ζ cells (black columns) were stimulated at 5µg/mL with the indicated plate-bound antibodies for 18 h and GFP induction was assayed by flow cytometry. Columns represent the mean ± s.d.; n=3. C FCRL6ζ cells were stimulated as in panel (B) with different concentrations of plate-bound anti-FCRL6 mAb. The percentage of GFP+ 43-1 cells is indicated in each histogram and demarcated by horizontal bars.