Table 1.

Target cDNA primer sequences used for real-time PCR

COL1

Forward: 5′-GAGGGCCAAGACGAAGACATC-3′

Reverse: 5′-CAGATCACGTCATCGCACAAC-3′

RUNX2

Forward: 5′-TGGTTACTGTCATGGCGGGTA-3′

Reverse: 5′-TCTCAGATCGTTGAACCTTGCTA-3′

OPN

Forward: 5′-CAGTTGTCCCCACAGTAGACAC-3′

Reverse: 5′-GTGATGTCCTCGTCTGTAGCATC-3′

OCN

Forward: 5′-CCCAGGCGCTACCTGTATCAA-3′

Reverse: 5′-GGTCAGCCAACTCGTCACAGTC-3′

GUSB (housekeeping gene)

Forward: 5′-GTCTGCGGCATTTTGTCGG-3′

Reverse: 5′-CACACGATGGCATAGGAATGG-3′

Abbreviations: cDNA, complementary DNA; PCR, polymerase chain reaction; COL1, collagen type Iα1; RUNX2, runt-related transcription factor 2; OPN, osteopontin; OCN, osteocalcin; GUSB, glucuronidase, beta.