Fig. 6.

FxFP peptides interact with the Cbk1 kinase domain. Fragments from proteins identified in the FxFP cluster were expressed as MBP fusions and immobilized on amylose resin. The beads were assayed for binding to GST-tagged Cbk1 (Cbk1^Δ1-351) in a pull-down assay. Binding was detected by Western blot for all six protein fragments tested, while MBP alone could not pull down Cbk1 (lower panel). Shown is a representative blot from three independent experiments. See fig. S6 for a shorter exposure of the blot that shows the loading control.