Figure 1. Criteria to Establish Human Risk from Developmental EDC Exposures.

Cross species comparisons are necessary at each stage of experimental design from critical periods of exposure to dose to adverse outcome measurements to establish weight of evidence for translation to human health and determination of human risk of disease. Using animal models that have similar, measureable adverse health outcomes, such as sensitive measure of oxidative stress, in the pathway to chronic disease development are important for understanding disease development and progression. Mediation of oxidative stress is shown via arrows A and B. In order for oxidative stress to be a mediator, it must be significantly associated with both EDC dose and chronic disease outcome and must be in the in the pathway from exposure to disease.