. Author manuscript; available in PMC: 2016 May 23.

Published in final edited form as: Diabetes. 2016 Jan 6;65(4):851–860. doi: 10.2337/db15-1531

Table 2.

The effect of pregnancy and gestational age on the biometry and biochemical composition in non-pregnant females and pregnant dams at day (D)16 and D19 of pregnancy.

	Non-pregnant	Pregnant

		D16	D19
Biometry
Total body weight BW (g)	21.0 ± 0.4^a (20)	31.0 ± 1.1^b (13)	35.0 ± 1.2^b (16)
Hysterectomised weight HW (g)	-	24.0 ± 0.7^b (13)	23.0 ± 0.8^b (15)
Liver (mg) (% )^†	1099 ± 28^a (10)	1903 ± 57^b (13)	1856 ± 76^b (16)
Liver (mg) (% )^†	5.5 ± 0.2^a (10)	7.9 ± 0.1^b (13)	8.1 ± 0.3^b (16)
Kidney (mg) (% )^†	234.0 ± 6.5^a (10)	277 ± 12^b (11)	271 ± 11^b (15)
Kidney (mg) (% )^†	1.20 ± 0.02 (10)	1.10 ± 0.02 (11)	1.20 ± 0.04 (15)
Heart (mg) (% )^†	106.0 ± 6.5^a (10)	132 ± 5^b (13)	137 ± 7^b (16)
Heart (mg) (% )^†	0.50 ± 0.03 (10)	0.50 ± 0.01 (13)	0.60 ± 0.03 (16)
Retroperitoneal fat (mg) (%)^†	30 ± 2^a (16)	73 ± 7^b (13)	63 ± 6^b (16)
Retroperitoneal fat (mg) (%)^†	0.1 ± 2.0^a (16)	0.30 ± 0.03^b (13)	0.30 ± 0.03^b (16)
Gravid uterus (g)	-	7.3 ± 0.4 (13)	11.2 ± 0.7^* (15)
Placenta average per litter (mg)	-	104.0 ± 1.8 (13)	88.0 ± 2.7^* (16)
Fetus average per litter (mg)	-	427 ± 12 (13)	1204 ± 17^* (16)
Litter size	-	7.8 ± 0.5 (13)	7.1 ± 0.4 (16)
Biochemical composition
DEXA absolute fat mass (g)^†	3.9 ± 0.2 (10)	4.4 ± 0.3 (7)	4.5 ± 0.2 (7)
DEXA fat mass (%)^†	17.6 ± 0.9 (10)	17.8 ± 1.2 (7)	19.2 ± 0.5 (7)
DEXA absolute lean mass (g)^†	18.2 ± 0.6^a (10)	20.3 ± 0.5^b (7)	18.7 ± 0.7^ab (10)
DEXA lean mass (%)^†	82.4 ± 0.9 (10)	82.3 ± 1.2 (7)	80.9 ± 0.5 (10)
Hepatic glycogen (mg/g)	55 ± 3 (15)	49 ± 1 (6)	57 ± 6 (6)
Total hepatic glycogen (mg)	61 ± 4^a (15)	86 ± 3^b (6)	103 ± 11^b (6)
Hepatic fat content (%)	5.4 ± 0.3 (15)	5.1 ± 0.3 (6)	4.7 ± 0.3 (6)
Total hepatic fat content (mg)	59 ± 5^a (15)	91 ± 6^b (6)	86 ± 6^b (6)
Skeletal muscle fat content (%)^§	4.0	15.4	20.4

Data are expressed as mean ± SEM with the number of dams/litters in parentheses. Values with different superscripts are significantly different from each other (p<0.05, one way ANOVA).

significant difference between D16 and D19 pregnant dams (p<0.05, t-test).

^†

Organ weights and DEXA results were expressed as % of total body weight for NP females and of hysterectomised for pregnant dams.

^§

skeletal muscle fat content was measured on samples pooled from 3-5 animals from each group.