Table 2.
Combination index (±standard deviation) and combinatorial profiles for different antiretrovirals (ACV and TFV) and G2-S16, G1-S4, and G3-S16 dendrimer combinations at equipotent ratio against HSV-2 strain 333 isolate in Vero cells
| Dendrimer | ARV | Combination ratio | CI
|
||
|---|---|---|---|---|---|
| EC50 | EC75 | EC90 | |||
| G2-S16 | ACV | 1:1 | 1.055±0.122 (±) |
0.705±0.053 (++) |
0.528±0.041 (+++) |
| TFV | 1:1 | 1.305±1.147 (−) |
1.013±0.702 (±) |
0.843±0.455 (++) |
|
| G1-S4 | ACV | 1:1 | 0.912±0.103 (±) |
0.616±0.139 (+++) |
0.442±0.146 (+++) |
| TFV | 1:1 | 0.976±0.244 (±) |
0.826±0.282 (++) |
0.754±0.315 (++) |
|
| G3-S16 | ACV | 1:10 | 0.852±0.160 (+) |
0.767±0.231 (+) |
0.911±0.596 (±) |
| TFV | 1:20 | 1.108±0.415 (−) |
0.792±0.369 (++) |
0.597±0.286 (+++) |
|
Notes: CI calculated at the EC50, EC75, and EC90 level. CI <0.9 indicates synergism; 0.9< CI <1.1 indicates additive effects, and CI >1.1 indicates antagonism (−). Synergy level: 0.85< CI <0.9 + (slight synergism); 0.7< CI <0.85 ++ (moderate synergism); 0.3< CI <0.7 +++ (synergism). None of the results have the values of 0.1<Cl<0.3 ++++ (potent synergism); 0.1> CI +++++ (very strong synergism). Data are represented as mean ± standard deviation of three independent experiments.
Abbreviations: ACV, acyclovir; ARV, antiretroviral; CI, combination index; TFV, tenofovir.