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. Author manuscript; available in PMC: 2016 May 23.
Published in final edited form as: Endocr J. 2015 Mar 11;62(4):379–386. doi: 10.1507/endocrj.EJ14-0449

Fig. 3.

Fig. 3

WS6 does not adversely affect human islet viability. Analysis of human islets exposed to WS6 1.0 μM or DMSO (control) for 96 hours.

A. Viability of human islet cells as assayed by flow cytometry Live/Dead assay. Gating strategy (left) and quantification of viable cells (right, n = 3 from 3 different donors, p = NS.) B. Analysis of apoptosis by immunofluorescent TUNEL assay. Left - Arrows indicate TUNEL+ cells (Green = TUNEL staining, Blue = DAPI). Right – Quantification of TUNEL+ cells per islet (n = 3 from 3 different donors, p = NS). (Bars indicate mean ± SEM. Scale bar = 50 μm)