Extended Data Figure 3. Gating strategy for flow cytometry studies in Guinea.
All samples evaluated in the field were aliquoted for four panels. All panels had the following common gating: G1: Lymphocyte gate; G2: Live cells; G3: Singlets; G4: T cells; G5: CD4 T cells; G6: CD8 T cells. Panel 1 Panel 1, 2, 3, and 4 evaluated expression of HLA-DR, Ki-67, CTLA-4, and PD-1, respectively, in either CD4 or CD8 T cells.