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. 2016 Jan 25;2:15069. doi: 10.1038/cddiscovery.2015.69

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Copyright © 2016 Cell Death Differentiation Association

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Blebbishields rapidly internalize fusogenic membranes and mitotic cells fuse to each other using surface-exposed Golgi membranes. (a) Live blebbishields internalized labeled surface membranes (arrowheads), but fixed blebbishields did not. Note the labeling is restricted to the surface (arrows). DIC, differential interference contrast. (b) Nocodazole-arrested mitotic cells did not internalize surface membranes. (c) Genetic labeling of Golgi membranes using Golgi-CFP revealed that they were exposed to the cell surface (arrows) during metaphase and telophase in untreated 293T mitotic cells and underwent fusion during telophase. CF, mitotic cleavage furrow; FF, fusion furrow. DIC, differential interference contrast. (d) 293T mitotic cells were capable of fusion with blebbishields derived from RT4P cells. n, number of colonies.