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. 2016 Jan 25;2:15069. doi: 10.1038/cddiscovery.2015.69

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Copyright © 2016 Cell Death Differentiation Association

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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VEGFR2 undergoes endocytosis in blebbishields, and internalized VEGFR2 colocalizes with PKH26-labeled lipid membranes in transformed spheres. (a and b) Double immunofluorescence analysis of VEGFR2 and syntaxin-6 (trans-Golgi-network marker) showed their co-localization at the perinuclear trans-Golgi-network (arrows) in non-apoptotic cells (a) Immunofluorescence analysis of VEGFR2 and syntaxin-6 and in freshly fixed blebbishields; internalized VEGFR2 is marked by arrows (b). (c) Lipo-immunofluorescence analysis of VEGFR2 and syntaxin-6 co-localization with PKH26-labeled blebbishield surface membrane lipids (internalized in spheres; co-localization marked by arrows). DIC, differential interference contrast.