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. Author manuscript; available in PMC: 2016 May 23.
Published in final edited form as: Botanics. 2015 Nov 30;5:65–76. doi: 10.2147/BTAT.S62984

Figure 4.

Figure 4

Multiple-solvent fractions of Vernonia amygdalina (VA) extracts inhibited DNA synthesis.

Notes: Cells at the logarithmic growth phase were treated with either 100 or 1,000 μg/mL for 18 hours before the addition of 1 μCi/mL [3H]thymidine for 6 hours. Each data point represents the mean of three independent experiments done in duplicates (N=6). Exposure of cells to multiple-solvent fractions (butanol, chloroform, ethyl acetate, hexane, and methanol) of VA inhibited DNA synthesis in both concentration and solvent-dependent fashion. *Hexane, chloroform, and ethyl acetate extracted fractions (A-1, A-2, and B-3) at 100 μg/mL inhibited DNA synthesis by approximately 30%. **One thousand microgram per milliliter of fractions of hexane, chloroform, butanol, and ethyl acetate (A-1–3, B-2, B-3, and B-4) inhibited DNA synthesis by 76%, 98%, 94%, 98%, 98%, and 96% (P<0.001), respectively [3H]thymidine uptake was determined.

Abbreviations: cpm, counts per minute; Cont, control.