Table 1. RT and heights of two characteristic peaks in HPLC fingerprints of four fraction extracts of a batch of VA.
VA fraction no | Peak 1 RT (min) | Peak 2 RT (min) | Mean RT (min) | SD RT (min) | Peak 1 height (mAU) | Peak 2 height (mAU) | Mean height (mAU) | SD height (mAU) |
---|---|---|---|---|---|---|---|---|
4 | 2.206 | 2.208 | 2.207 | 0.001 | 5.50 | 14.0 | 9.75 | 6.010 |
A-2 | 2.210 | 2.213 | 2.2115 | 0.002 | 1.2 | 3.5 | 2.35 | 1.626 |
B-2 | 2.185 | 2.187 | 2.186 | 0.001 | 6.9 | 25.0 | 15.95 | 12.799 |
B-3 | 2.150 | 2.152 | 2.151 | 0.001 | 2.25 | 6.7 | 4.475 | 3.147 |
Notes: All fraction peaks expressed at a relatively similar retention time, while higher peak height was expressed in fractions 4 and B-3. Fingerprint experiment was conducted in duplicates, and mean and standard deviations for peak height and retention time computed.
Abbreviations: RT, retention time; VA, Vernonia amygdalina; SD, standard deviation; HPLC, high performance liquid chromatography.