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. 2016 Apr 2;37(6):589–599. doi: 10.1093/carcin/bgw039

Figure 2.

Figure 2.

Investigating the capacity of CCM to increase the invasiveness and clonogenicity of PCA cells. (A) Basal media, CCM or SBCM were placed in the bottom wells of Trans-well invasion chambers, and WPE-1 NA-22, WPE-1 NB-14 and PC3 cells were seeded onto the top chamber. Cells were allowed to invade for 18h and then fixed and imaged. Cells were quantified across five randomly selected fields at 400× magnification with three replicates (*P ≤ 0.05 from basal and CCM, respectively). (B) RWPE-1, (C) WPE-1 NB-14 and (D) PC3 cells were incubated in basal media, CCM, SBCM or CCM with silibinin (50 µM) for 8 days. Cells were then fixed and imaged for colony formation. Colonies consisting of >50 cells were counted. Data shown in bar diagram represent mean ± SEM of three samples for each group (*P ≤ 0.05).