Table 2. Differential expression of known gma-miRNAs in soybean seeds.
| Sequence (5’-3’) | Mature miRNA | Log2(MON/A32) | Log2(DP3/JAC) | Log2(A32/JAC) | Same to gma-miRNAs | |||
|---|---|---|---|---|---|---|---|---|
| TCTCATTCCATACATCGTCTGA | miR1507a | -1.03 | ↓ | 0.49 | — | 0.88 | — | gma-miR1507a |
| AGGGATAGGTAAAACAACTACT | miR1510b-5p | -1.10 | ↓ | -0.34 | — | 0.98 | — | gma-miR1510b-5p |
| AACCAGGCTCTGATACCATG | miR1511 | 0.06 | — | 2.59 | ↑ | 2.07 | ↑ | gma-miR1511 |
| TTGACAGAAGATAGAGAGCAC | miR156c | -0.44 | — | 0.70 | — | 1.07 | ↑ | gma-miR156c,d,e,i,j,l,m |
| TCGGACCAGGCTTCATTCCCC | miR166a-3p | -1.10 | ↓ | 0.44 | — | 1.00 | ↑ | gma-miR166a-3p,b,c-3p,d,e,f,g,n,o,i-3p |
| TCTCGGACCAGGCTTCATTCC | miR166h-3p | -1.29 | ↓ | 0.66 | — | 1.46 | ↑ | gma-miR166h-3p,k |
| TCTCGGACCAGGCTTCATTC | miR166u | -1.36 | ↓ | 0.41 | — | 0.95 | — | gma-miR166u |
| GGAGATGGGAGGGTCGGTAAAG | miR2118a-5p | -0.38 | — | -0.46 | — | -1.10 | ↓ | gma-miR2118a-5p,b-5p |
| TTGGACTGAAGGGAGCTCCC | miR319a | -1.21 | ↓ | 0.62 | — | 1.21 | ↑ | gma-miR319a,b,e |
| AAGCTCAGGAGGGATAGCGCC | miR390a-5p | -1.94 | ↓ | 0.21 | — | 1.58 | ↑ | gma-miR390a-5p,f,g |
| AGCTCAGGAGGGATAGCGCC | miR390e | -1.77 | ↓ | 0.31 | — | 2.00 | ↑ | gma-miR390e |
| TTCCACAGCTTTCTTGAACTT | miR396b-5p | -1.00 | ↓ | 0.30 | — | 0.88 | — | gma-miR396b-5p,c |
| TGTGTTCTCAGGTCGCCCCTG | miR398c | -0.42 | — | 2.49 | ↑ | 2.79 | ↑ | gma-miR398c |
| TGTTGCGGGTATCTTTGCCTC | miR4412-5p | 0.36 | — | -0.12 | — | -1.57 | ↓ | gma-miR4412-5p |
| TCTTCCCTACACCTCCCATACC | miR482b-3p | -1.27 | ↓ | 0.21 | — | 1.27 | ↑ | gma-miR482b-3p,d-3p |
| TTCCCAATTCCGCCCATTCCT | miR482c-3p | -0.93 | — | -1.17 | ↓ | 1.26 | ↑ | gma-miR482c-3p |
| TGAAGATTTGAAGAATTTGGGA | miR5376 | -0.46 | — | -0.36 | — | -1.19 | ↓ | gma-miR5376 |
MON, MON89788; A32, A3244; DP3, DP-3Ø5423×GTS 40-3-2; JAC, Jack. gma-miRNAs with original abundance more than 100 reads were listed. Log2 ratios of normalized gma-miRNA reads from GM and non-GM soybean seeds were calculated, P < 0.001.