Table 2. Kinetic characterizzation of Ma4CL3.
Isoenzyme Substrate | Km (μM) | Vmax (nKat mg−1) | Kcat (s−1) | K cat / K m (μM−1 s−1) |
---|---|---|---|---|
Cinnamic acid | 73.53 | 22.15 | 1.041 | 0.014 |
4-Coumarate acid | 10.49 | 4.40 | 0.284 | 0.027 |
Caffeate acid | 463.69 | 16.15 | 1.428 | 0.003 |
Ma4CL3 was no catalytic activity to Sinapate acid and ferulate acid.