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. 2016 May 23;11(5):e0155951. doi: 10.1371/journal.pone.0155951

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© 2016 Swartzwelder et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — (A) Adolescent Intermittent Ethanol (AIE) or Saline (AIS) Exposure: Rats received intragastric (i.g.) 5g/kg ethanol or saline every other day from postnatal day (P) 30–46. (B) The hippocampus was dissected from brains collected in adulthood between P70-75 (8 AIE & 8 AIS; two rats/sample). (C) Subcellular Fractionation. Synaptic-enriched/S3 [triton- & deoxycholate (DOC)-soluble] and non-synaptic/S2 (triton-soluble) fractions were generated. (D) GluN2B Immunoprecipitation (IP) was performed on S2 and S3 fractions from AIE and AIS treatments. (E) Mass spectrometry identified 696 proteins across all samples.