Fig 1. Comparative genomic hybridization on a female karyotype of Pseudis tocantins.

(A-B) Using female genomic probe labeled with Cy-3 (red), male derived probe labeled with FITC (green), male unlabeled competitor DNA and DAPI counterstaining, CGH detected a Cy-3 signal in the W chromosome. In (A), DAPI-stained chromosomes are shown. (C) ZW pair after C-banding and DAPI staining. Note the heterochromatic bands in the Z and W chromosomes. Bar: 5 μm.