PcP190 sequences isolated from Pseudis tocantins aligned with PcP190 sequences of Physalaemus cuvieri (JF281121.1, KM361677.1, KM361682.1, KM361675.1), Physalaemus centralis (KM361684.1 and KM361685.1), Physalaemus albonotatus (KM361689.1 and KM361690.1), Physalaemus albifrons (KM361694.1, KM361696.1 and KM361698.1), Physalaemus ephippifer (KM361699.1 and KM361700.1), Physalaemus marmoratus (KM361701.1 and KM361702.1), Leptodactylus latrans (KM361718.1 and KM361719.1) and Crossodactylus gaudichaudii (KM361725.1 and KM361726.1). Annealing regions of Primer P190F and P190R are indicated with green arrows. Sequences of PcP190 from P. tocantins isolated from genomic DNA (■), microdissected W (*) and Z (#) chromosomes are indicated.