Table 1. Genetic similarity (%) between the presumed transcribed regions of the 5S rDNA of Pseudis tocantins and those from several anurans obtained from GenBank.

	P. tocantins type I 5S rDNA	P. tocantins type II 5S rDNA
Anaxyrus americanusa	81.36 (±4.41)	79.39 (±4.48)
Engystomops freibergi and E. petersi type I 5S rDNAb	79.03 (±4.62)	78.96 (±4.62)
Engystomops freibergi and E. petersi type II 5S rDNAc	70.83 (±5.04)	82.68 (±4.30)
Gastrotheca riobambaed	78.73 (±4.64)	82.02 (±4.23)
Lithobates pipiens and L. catesbeianuse	77.74 (±4.59)	76.75 (±4.55)
Pelophylax lessonae and P.ridibundusf	79.50 (±4.60)	80.18 (±4.52)
Physalaemus cuvieri type I 5S rDNAg	78.51 (±4.73)	79.17 (±4.60)
Physalaemus cuvieri type II 5S rDNAh	67.76 (±5.26)	82.31 (±4.20)
Xenopus borealisi	80.04 (±4.43)	74.12 (±4.85)
Xenopus laevis and X. tropicalis somatic 5S RNA genej	77.74 (±4.60)	80.04 (±4.39)
Xenopus laevis and X. tropicalis oocytic 5S RNA genek	75.98 (±4.61)	77.31 (±4.38)
Pseudis tocantins type I 5S rDNAl	-	64.69 (±5.41)
Pseudis tocantins type II 5S rDNAm	64.69 (±5.41)	-

Numbers in parentheses indicate the standard error. The regions corresponding to the primers 5S-A and 5S-B (used to isolate the 5S rDNA sequences of P. tocantins) were not considered in this comparative analysis.

^aAnaxyrus americanus 5S rRNA gene—X58365.1 (as Bufo americanus)

^bEngystomops freibergi type I 5S rRNA gene—JF325868.1-JF325870.1, Engystomops petersi type I 5S rRNA gene—JF325859.1—JF325867.1

^cE. petersi type II 5S rRNA gene—JF325846.1-JF325858.1, E. freibergi type II 5S rRNA gene—JF325843.1-JF325845.1

^dGastrotheca riobambae 5S rRNA gene—M74438.1

^eLithobates pipiens 5S rRNA gene—X58368.1; Lithobates catesbeianus 5S rRNA gene—X58367.1

^fPelophylax lessonae 5S rRNA gene—FJ572051.1; Pelophylax ridibundus 5S rRNA gene—FJ572052.1

^gPhysalaemus cuvieri type I 5S rDNA—JF281126.2-JF281131.2

^hP. cuvieri type II 5S rRNA gene—JF281132.2-JF281134.2

ⁱXenopus borealis 5S rRNA gene—V01425.1

^jXenopus laevis somatic 5S rRNA gene—J01009.1, Xenopus tropicalis somatic 5S rRNA gene—X12622.1 and NR 033271.1

^kX. laevis oocyte specific 5S rRNA gene—J01010.1, J01012.1, M10635.1, M63899.1, X05089.1, X. tropicalis oocyte specific 5S rRNA gene -NR_033270.1, NR_033271.1, X12623.1, X12624.1

^lsequences of type I 5S rDNA of Pseudis tocantins from this study

^msequences of type II 5S rDNA from P. tocantins from this study.