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. 2016 May 23;11(5):e0156106. doi: 10.1371/journal.pone.0156106

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© 2016 Luo et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — (A) SEC-HPLC analysis of the purity and estimation of the molecular-weight of AK-TNFα and TNFα. (B) Analysis of the biological function (L929 assay) and determination of the IC50 of AK-TNFα and cleaved TNFα in comparison to a commercial source of TNFα (R&D Systems). Data presented were representative of at least three independent experiments.