Table 1. Details of primers used in assays for the isoforms of chick TGF-βs and TGF-β receptors.

Gene	NCBI Access Number	Sequences (5’-3’)	Efficiency	Amplicon
TGF-β1	JQ423909.1	Forward: 5’-ACATCGACTTCCGCAAGGAT-3’	100%	69 bp
		Reverse: 5’-AGAAGTTGGCCATATAACCTTTGG- 3’
TGF-β2	NM_001031045.2	Forward: 5’-GCTGCGTGTCCCAGGATTT-3’	95.1%	60 bp
		Reverse: 5’-TGGGTGTTTTGCCAATGTAGTAGA-3’
TGF-β3	NM_205454.1	Forward: 5’-TGATGATGCTACCCCCACATC-3’	99.4%	141 bp
		Reverse: 5’-CCTGTCGGAAGTCAATGTAAAGAG-3’
TGFBR1	NM_204246.1	Forward: 5’- CAATTCCAACACCAGGTCCTACT-3’	100%	66 bp
		Reverse: 5’- CTGCCAGTTCCACAGGTCCTA-3’
TGFBR2	NM_205428.1	Forward: 5’-CAGATGTGTACTCCATGGCTTTG-3’	100%	82 bp
		Reverse: 5’-GGCTCATACTCTTTCACTTCTCCAA-3’
TGFBR3	NM_204339.1	Forward: 5’-CTGCAGGCAAGCGGCTATAC-3’	100%	68 bp
		Reverse: 5’-TTCATCCTGGCTTTGCAAGA-3’