Table 1. The PLD genes in G. hirsutum and G. raimondii and properties of the deduced proteins.
| Gene name | Gene ID | Chromosomal locationa | ORF(bp) | Proteinb | |||
|---|---|---|---|---|---|---|---|
| Size (aa) | MW (kDa) | pI | Subcellular localizationc | ||||
| GhPLDα1A | Gh_A10G0662 | A10(-): 11048194–11066260 | 2,424 | 807 | 91.53 | 5.37 | C |
| GhPLDα1D | Gh_D10G0730 | D10(+): 8439309–8443570 | 2,424 | 807 | 91.53 | 5.48 | C |
| GhPLDα2A | Gh_A06G1624 | A06(-): 101165820–101169178 | 2,418 | 805 | 91.6 | 5.5 | C |
| GhPLDα2D | Gh_D06G1993 | D06(-): 61526855–61530236 | 2,418 | 805 | 91.55 | 5.55 | C |
| GhPLDα3A | Gh_A07G0222 | A07(+): 2700343–2703900 | 2,484 | 827 | 93.99 | 6.65 | C |
| GhPLDα3D | Gh_D07G0279 | D07(+): 2893589–2897211 | 2,484 | 827 | 94.07 | 6.76 | C |
| GhPLDα4A | Gh_A05G0436 | A05(-): 4839584–4842566 | 2,454 | 817 | 92.9 | 6.71 | C |
| GhPLDα4D | Gh_D05G0560 | D05(+): 4511885–4514866 | 2,454 | 817 | 93.08 | 6.62 | C |
| GhPLDβ1A | Gh_A11G0758 | A11(+): 7513190–7519138 | 3,375 | 1,124 | 125.68 | 7.7 | N |
| GhPLDβ1D | Gh_D11G0886 | D11(+): 7677837–7683822 | 3,375 | 1,124 | 125.53 | 7.2 | N |
| GhPLDβ2A | Gh_A12G0942 | A12(+): 59715352–59718967 | 2,355 | 784 | 88.99 | 8.16 | C |
| GhPLDβ2D | Gh_D12G1032 | D12(+): 36125870–36130292 | 3,168 | 1,055 | 119.57 | 8.28 | N |
| GhPLDβ3A | Gh_A11G0761 | A11(+): 7538255–7544383 | 3,390 | 1,129 | 125.42 | 6.7 | N |
| GhPLDβ3D | Gh_D11G0888 | D11(+):7700913–7707032 | 3,393 | 1,130 | 125.44 | 6.63 | N |
| GhPLDγ A | Gh_A08G1600 | A08(+): 94594467–94598013 | 2,556 | 851 | 95.54 | 8.19 | C |
| GhPLDγD | Gh_D08G1911 | D08(+): 57054075–57057621 | 2,556 | 851 | 95.9 | 7.6 | C |
| GhPLDδ1A | Gh_A12G0540 | A12(-): 13280804–13286284 | 2,550 | 914 | 96.46 | 6.69 | C |
| GhPLDδ1D | Gh_D12G0556 | D12(-): 10278949–10284459 | 2,550 | 914 | 96.37 | 6.69 | C |
| GhPLDδ2A | Gh_A02G1674 | A02(+):83053172–83057736 | 2,415 | 804 | 90.98 | 7.18 | C |
| GhPLDδ2D | Gh_D03G0048 | D03(+):312090–316688 | 2,571 | 856 | 96.97 | 6.63 | C |
| GhPLDδ3A | Gh_A01G0880 | A01(-):20697344–20701722 | 2,538 | 845 | 95.68 | 6.64 | C |
| GhPLDδ3D | Gh_D01G0915 | D01(-):15285684–15289947 | 2,538 | 845 | 95.78 | 6.72 | C |
| GhPLDδ4A | Gh_A05G2168 | A05(-):24844619–24854267 | 3,693 | 1,230 | 140.562 | 8.7 | N |
| GhPLDδ4D | Gh_D05G2424 | D05(-):24298286–24302113 | 2,595 | 864 | 98.669 | 8.44 | N |
| GhPLDδ5A | Gh_A12G0243 | A12(-):3722040–3726903 | 2,547 | 848 | 96.03 | 6.79 | C |
| GhPLDδ5D | Gh_D12G0242 | D12(-):3218723–3223600 | 2,550 | 849 | 96.12 | 7.03 | C |
| GhPLDε A | Gh_A02G1124 | A02(-):60940992–60943829 | 2,307 | 768 | 87.87 | 6.45 | C |
| GhPLDεD | Gh_D03G0546 | D03(-):10422279–10425127 | 2,307 | 768 | 87.72 | 6.75 | C |
| GhPLDζ1A | Gh_A01G0213 | A01(+):2083336–2091108 | 3,321 | 1,106 | 125.96 | 6.1 | C |
| GhPLDζ1D | Gh_Sca005308G01 | scaffold5308(+):923–8640 | 3,321 | 1,106 | 126.01 | 6.1 | C |
| GhPLDζ2A | Gh_A10G1486 | A10(+):81489677–81498158 | 3,345 | 1,114 | 126.71 | 6.33 | N |
| GhPLDζ2D | Gh_D10G1729 | D10(+):48605329–48613791 | 3,345 | 1,114 | 126.49 | 6.31 | N |
| GhPLDζ3A | Gh_A02G0494 | A02(+):7274495–7288655 | 2,919 | 972 | 109.9 | 6.28 | N |
| GhPLDζ3D | Gh_D02G0554 | D02(+):7493511–7502060 | 2,946 | 981 | 111.07 | 6.28 | N |
| GhPLDζ4A | Gh_A11G2458 | A11(+):83020505–83026075 | 3,327 | 1,108 | 126.32 | 5.57 | C |
| GhPLDζ4D | Gh_D11G2775 | D11(+):57479136–57489254 | 3,192 | 1,063 | 121.3 | 5.56 | C |
| GhPLDφ1A | Gh_A07G0265 | A07(-):3306792–3314618 | 1,545 | 514 | 57.91 | 7.23 | E |
| GhPLDφ1D | Gh_D07G0321 | D07(-):3400982–3403213 | 1,545 | 514 | 57.94 | 7.99 | E |
| GhPLDφ2A | Gh_A06G0922 | A06(-):37290132–37292468 | 1,380 | 459 | 52.17 | 8.98 | PM |
| GhPLDφ2D | Gh_D06G1085 | D06(-):24321096–24323423 | 1,623 | 540 | 61.55 | 9.13 | PM |
| GrPLDα1 | Gorai.011G083000.1 | Chr11(+): 8380923–8385184 | 2,424 | 807 | 91.58 | 5.44 | C |
| GrPLDα2 | Gorai.010G225600.1 | Chr10(-):59751136–59754500 | 2,418 | 805 | 91.51 | 5.63 | C |
| GrPLDα3 | Gorai.001G033300.1 | Chr1(+):3069538–3073522 | 2,484 | 827 | 93.88 | 6.33 | C |
| GrPLDα4 | Gorai.009G057800.1 | Chr9(+):4158704–4161686 | 2,454 | 817 | 93.14 | 6.55 | C |
| GrPLDβ1 | Gorai.007G094300.1 | Chr7(+):6927130–6933259 | 3,393 | 1130 | 125.61 | 6.73 | N |
| GrPLDβ2 | Gorai.008G115700.1 | Chr8(+):34857730–34862153 | 3,162 | 1053 | 119.23 | 7.59 | N |
| GrPLDβ3 | Gorai.007G094100.1 | Chr7(+):6908403–6914399 | 3,375 | 1124 | 125.64 | 7.51 | N |
| GrPLDγ | Gorai.004G206700.1 | Chr4(+):53722628–53726174 | 2,556 | 851 | 95.93 | 7.6 | C |
| GrPLDδ1 | Gorai.008G061600.1 | Chr8(-):9809029–9814536 | 2,550 | 849 | 96.25 | 6.69 | C |
| GrPLDδ2 | Gorai.003G005500.1 | Chr3(-):302357–306946 | 2,565 | 854 | 96.8 | 6.55 | C |
| GrPLDδ3 | Gorai.002G120800.1 | Chr2(-):17310003–17314270 | 2,562 | 853 | 96.72 | 6.59 | C |
| GrPLDδ4 | Gorai.009G268500.1 | Chr9(-):22371067–22374881 | 2,523 | 840 | 95.74 | 8.6 | PM |
| GrPLDδ5 | Gorai.008G027300.1 | Chr8(-):3218926–3223804 | 2,550 | 849 | 96.14 | 6.91 | C |
| GrPLDε | Gorai.003G059700.1 | Chr3(-):10351275–10354153 | 2,307 | 768 | 87.6 | 6.67 | C |
| GrPLDζ1 | Gorai.002G028800.1 | Chr2(+):2142619–2150335 | 3,321 | 1106 | 125.91 | 6.21 | C |
| GrPLDζ2 | Gorai.011G193900.1 | Chr11(+):46880104–46888580 | 3,300 | 1099 | 124.6 | 6.15 | C |
| GrPLDζ3 | Gorai.005G062100.1 | Chr5(+):6558010–6566183 | 3,273 | 1090 | 124.04 | 6.18 | N |
| GrPLDζ4 | Gorai.007G302800.1 | Chr7(+):51639889–51645452 | 3,237 | 1078 | 123.16 | 5.58 | C |
| GrPLDφ1 | Gorai.001G038100.1 | Chr1(-):3551503–3553734 | 1,545 | 514 | 58 | 7.19 | E |
| GrPLDφ2 | Gorai.010G118800.1 | Chr10(-):23679895–23682222 | 1,545 | 514 | 58.28 | 8.96 | E |
a. Chromosomal location: [ChrNo.(Orientation): start-end], ‘+’ and ‘-’ indicated the forward and reverse orientation, respectively.
b. The theoretical molecular weight (MW) and isoelectric point (pI) were calculated by ExPASy. Subcellular localization was analyzed using the CELLO v2.5 server. The Nucleic acid and deduced amino acid sequences of each GhPLD and GrPLD gene are listed in S2 Table.
c. C, cytoplasm; N, nucleus; PM, plasma membrane; E, extracellular space.