Table 1. Origins offield collected cotton samples and presence of viruses.
| Location | Isolate | GPS coordinates | Virus* | |||
|---|---|---|---|---|---|---|
| Province | District | North | East | ToLCNDV | CLCuKoV | |
| Punjab | Bahawalpur | C140 | 29.59636 | 72.83348 | - | ✓ |
| Punjab | Bahawalpur | C150 | 29.56129 | 72.74316 | - | ✓ |
| Punjab | Bahawalpur | K201 | 29.10163 | 71.7427 | ✓ | ✓ |
| Punjab | Bahawalpur | K202 | 29.10163 | 71.7427 | ✓ | ✓ |
| Punjab | Bahawalpur | K203 | 29.20806 | 71.75604 | ✓ | ✓ |
| Punjab | Bahawalpur | K204 | 29.20806 | 71.75604 | ✓ | ✓ |
| Punjab | Bahawalpur | K206 | 29.17652 | 71.86785 | - | ✓ |
| Punjab | Bahawalpur | K207 | 29.17652 | 71.86785 | ✓ | ✓ |
| Punjab | Bhakkar | D11 | 31.43663 | 71.52154 | - | ✓ |
| Punjab | Bhakkar | D26 | 31.48838 | 71.26929 | - | ✓ |
| Punjab | Faisalabad | C31 | 31.35432 | 73.30094 | - | ✓ |
| Punjab | Khanewal | H38 | 30.40681 | 72.12185 | - | ✓ |
| Punjab | Khanewal | H41 | 30.35511 | 72.08303 | - | ✓ |
| Punjab | Khanewal | H42 | 30.35511 | 72.08303 | - | ✓ |
| Punjab | Toba Tek Singh | H1 | 31.31402 | 72.79922 | - | ✓ |
| Punjab | Toba Tek Singh | H11 | 31.05338 | 72.58824 | - | ✓ |
| Punjab | Toba Tek Singh | H20 | 30.87548 | 72.53648 | ✓ | ✓ |
| Punjab | Toba Tek Singh | H9 | 31.05338 | 72.58824 | ✓ | ✓ |
| Punjab | Faisalabad | NIAB1 | 31.3982 | 73.0339 | ✓ | ✓ |
| Punjab | Faisalabad | NIAB2 | 31.3982 | 73.0339 | ✓ | ✓ |
| Sindh | Ghotki | K123 | 28.10269 | 69.71552 | ✓ | ✓ |
| Sindh | Nawabshah | SS23 | 26.1255 | 68.2804 | ✓ | ✓ |
| Sindh | Nawabshah | SS32 | 26.1255 | 68.2804 | ✓ | ✓ |
| Sindh | Nawabshah | SS33 | 26.1255 | 68.2804 | ✓ | ✓ |
| Sindh | Nawabshah | SS34 | 26.1255 | 68.2804 | ✓ | ✓ |
| Sindh | Nawabshah | SS35 | 26.1255 | 68.2804 | ✓ | ✓ |
| Sindh | Tando Allahyar | SS1 | 25.4799 | 68.7900 | ✓ | ✓ |
| Sindh | Mirpur Khas | SS10 | 25.5101 | 68.9301 | ✓ | ✓ |
| Sindh | Mirpur Khas | SS11 | 25.5101 | 68.9301 | ✓ | ✓ |
| Sindh | Hayderabad | SS16 | 25.4997 | 68.4196 | ✓ | ✓ |
| Sindh | Matiari | SS20 | 25.9299 | 68.3800 | ✓ | ✓ |
*The presence of either Tomato leaf curl New Delhi virus (ToLCNDV) or Cotton leaf curl Kokhran virus (CLCuKoV) was determined by diagnostic PCR with ToLCNDV DNA-B specific primers or with the CLCuKoV specific primers described by Shuja et al.[38].