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. 2016 May 23;11(5):e0156002. doi: 10.1371/journal.pone.0156002

Fig 7. ECIS barrier function assays showing the effect of Wnt5A in IL-4 induced hyperpermeability of HCAEC.

Fig 7

Uniform confluent monolayers of HCAEC cultured in stabilized and collagen coated ECIS 8W10E+ arrays were treated with IL-4. TEER of HCAEC monolayers was measured in Ohms continuously every 5 min at multiple frequencies ranging from 62.6 Hz to 64 kHz, normalized to its value at time zero and plotted with respect to time. Stimulations conducted in duplicate wells were grouped and averaged to plot a single curve with error bars representing mean ± SD. Figures shown depict the resistance measurements conducted at 4000 Hz and represent four independent experiments. (A) 1, non-treated Wnt5A-siRNA transfected HCAEC; 2, non-treated non-transfected HCAEC; 3, IL-4 treated Wnt5A-siRNA transfected HCAEC; 4, IL-4 treated non-transfected HCAEC. (B) 1, non-treated negative control siRNA transfected HCAEC; 2, IL-4 treated negative control siRNA transfected HCAEC; 3, IL-4 treated non-transfected HCAEC. (C) Black, non-treated; green, IL-6.