Skip to main content
. 2016 May 23;12(5):e1006069. doi: 10.1371/journal.pgen.1006069

Fig 4. S-pilin cleavage is necessary for transformation competence in pilE mutant strains.

Fig 4

A. Alignment of the PilE primary sequence surrounding the S-pilin cleavage site of the parental strain and the S-pilin cleavage mutant strains. B. PilE western blot of parental strain (CmR), ΔpilE mutant, pilE L38L39A40-AAM S-pilin cleavage mutant, a strain with P. aeruginosa PilA sequence at residues 37–43, and the S-pilin control mutation S45A46V47-TMA. Upper band is full-length pilin. Lower band is the processed S-pilin form. Western blot analysis performed using the K36 peptide anti-pilin antibody. C. Transformation efficiencies of a pilE C-terminal mutations coupled with the S-pilin cleavage site mutation (L38L39A40-AAM), P. aeruginosa pilA sequence (P.a. S-pil), or the control mutation (S45A46V47-TMA). X = nonsense mutation, CmR = CmR parental strain, *p<0.05 **p<0.001 Student’s T-test. ND = transformants not detected.