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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Exp Neurol. 2016 Apr 9;281:53–65. doi: 10.1016/j.expneurol.2016.04.006

Figure 6. Release of iCGRP is not altered in spinal cord slices from male rats with letrozole-induced mechanical hypersensitivity.

Figure 6

A, B, D and E: Basal and potassium-stimulated release of iCGRP was measured from spinal cord slices isolated from rats following treatment with vehicle (A, D) or 5 mg/kg letrozole (B, E) for 7–8 days. Open columns represent iCGRP released from spinal cord slices perfused with HEPES buffer alone for successive 3-minute intervals, whereas black columns represent iCGRP released when tissues were perfused with HEPES buffer containing 30 mM (A, B) or 50mM potassium (D, E). The ordinate represents the mean ± S.E.M. of iCGRP released per minute, expressed as the percent of total iCGRP content in the spinal cord slice. C and F: Summary of mean ± S.E.M. of iCGRP release as percent of total content/min during basal (minutes 9–18) and high potassium stimulation (minutes 18–27) in vehicle or letrozole-treated rats from (A - E). Evoked release is calculated as stimulated release minus basal release. Asterisks indicate significant differences in iCGRP release between stimulated and basal conditions using two-sample t-tests.