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. 2016 May 24;7:128. doi: 10.3389/fphar.2016.00128

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Copyright © 2016 Li, Chen, Liu, Lee, Man and Cheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bakuchiol induced apoptosis in MCF-7 cells. MCF-7 cells were treated with 0–10 μg/ml of bakuchiol for 24 h. (A) Morphology observation of bakuchiol treated MCF-7 under bright field. Small apoptotic bodies are labeled with red arrows. The white bar indicates 100 μm. (B) Representative flow cytometry results of annexin V/PI analysis. (C) JC-1 staining for analysis of mitochondrial membrane potential. Western blot analysis of expression levels of (D) Caspase family protein, including Cleaved Caspase 7, Cleaved Caspase 9, and Cleaved PARP, and (E) Bcl-2 family protein, including Bak, Bax, and Bim. β-Actin was used as loading control.