FIGURE 8.

Bakuchiol inhibited in vivo cell mass formation in zebrafish. (A) ∼300 MCF-7 cells were injected into the yolk sac of 48 hpf zebrafish embryos. 60 embryos were treated with ethanol (solvent control) or 0.5 μg/ml of bakuchiol for 72 h. Triplicates for each condition were performed. Representative bright field and fluorescent images of embryos in both control and treatment groups showing cell mass in the yolk sac area were shown. The black and white bars indicate 500 μm. (B) Statistical analysis of integrated fluroscent intensity of cell mass in control and treatment group (^∗p < 0.05, one sample t-test). Ctrl, control; BAK, bakuchiol.