Table 1.
Target | Primers | 5′-3′ | Annealing/extension temperatures | Reference |
---|---|---|---|---|
Bacteria + Archaea (SSU rRNA gene) | Uni340F | CCT ACG GGR BGC ASC AG | 55.8 | Takai and Horikoshi, 2000 |
Uni806R | GGA CTA CNN GGG TAT CTA AT | Takai and Horikoshi, 2000 | ||
Zetaproteobacteria (SSU rRNA gene) | Zeta672F | CGG AAT TCC GTG TGT AGC AGT | 58 | Kato et al., 2009 |
Zeta837R | GCC ACW GYA GGG GTC GAT ACC | Kato et al., 2009 | ||
Sulfate-reducing bacteria (SRB) (DsrA gene) | RH1-dsr-F | GCC GTT ACT GTG ACC AGC C | 55 | Ben-Dov et al., 2007 |
RH3-dsr-R | GGT GGA GCC GTG CAT GTT | Ben-Dov et al., 2007 | ||