Fig 2.
Thermal stability of Bet v 1 and mutant proteins 1 to 4 (Bet_mut1-4). A, Chemical denaturation was assessed by means of CD spectroscopy, and the fraction of denatured protein (Fdenatured) is displayed. B and C, Thermal denaturation was measured by using CD (Fig 2, B) and DSC (Fig 2, C). DSC data for wild-type Bet v 1 and mutants were run at a 60°/h scan rate and at 0.15 mg/mL (lower traces) and 0.5 mg/mL (upper traces) offset in the y-axis for clarity. D, Melting points calculated from DSC data.