Figure 4.

Cortical, growth plate, osteoblast, dynamic, and bone quality variables. Ten-day-old Clcn7^WT/WT and Clcn7^G213R/WT were treated with 4 mg/kg of scrambled- (SRC) or Clcn7^G213R-sticky siRNA jetPEI conjugate, three times a week for 4 weeks. At the end of the experiments, mice were sacrificed and their bone phenotype analyzed. (a) Cortical thickness (Cor.Th). (b) Growth plate width. (c) Osteoblast surface over bone surface (Ob.S/BS). (d) Histological images of osteoid (arrows). Bar = 5 µm. (e) Osteoid volume over bone volume (OV/BV). (f) Calcein labeling (green fluorescence) of mineral deposition (double arrowheads). Bar = 2 µm. (g) Mineral apposition rate (MAR). (h) Mineralized surface over bone surface (MS/BS). (i) Bone formation rate (BFR). (j) Total indentation distance (TDI). (k) First-cycle indentation distance (ID). (l) Touchdown distance (TDD). Results are (d,f) representative or (a–c,e,g–l) the mean ± SD of three to seven mice/group (Student's t-test). In a–c,e,g–i, P > 0.2. For a–c,e,g–l statistics was also performed by one-way analysis of variance (shown in Supplementary Table S3).