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. 2016 May 24;8:13. doi: 10.3389/fnsyn.2016.00013

Figure 2.

Figure 2

Analysis of presynaptic specializations after ProSAPiP1 knockdown in mature primary hippocampal neurons. (A) RNAi based knockdown of ProSAPiP1 in primary hippocampal culture (RNAi) leads to a significant reduction of endogenous ProSAPiP1, whereas there are no differences between the uninfected (Uninf.) and the scrambled control (Scr). PSD from rat hippocampus was loaded as reference for endogenous ProSAPiP1. β3-Tubulin and β-Actin serve as loading control. Statistical analysis was performed using One-way ANOVA. *p < 0.05; n = 3 lysates from three independent cultures for each condition. (B) Infected (GFP, green) primary hippocampal cultures were stained on DIV28 for Bassoon (red), VGluT1 (red) or VGAT (red) as indicated. No significant differences were observed in the density of Bassoon, VGluT1 or VGAT positive puncta per 10 μm dendrite length between Scr and RNAi. Scale bar: 10 μm. Statistical analysis was performed using unpaired two-sided t-test. n = 15 neurons from three independent cultures.