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. 2016 May 24;8:13. doi: 10.3389/fnsyn.2016.00013

Figure 3.

Figure 3

Analysis of Shank3 and SPAR after ProSAPiP1 overexpression and knockdown in mature primary hippocampal neurons. (A) Primary hippocampal cultures were infected with either FUGW empty vector (Vector) or GFP-ProSAPiP1 and stained for Shank3 (red) or SPAR (red) on DIV28 as indicated. The intensity of SPAR-positive puncta was significantly increased. (B) Primary hippocampal cultures were infected with either Scr or RNAi and stained for Shank3 (red) or SPAR (red) on DIV28 as indicated. A significant reduction was observed in both density and intensity of SPAR positive puncta between Scr and RNAi. Scale bar: 10 μm. Statistical analysis was performed using unpaired two-sided t-test. *p < 0.05; **p < 0.01. n = 15 neurons from three independent cultures.