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. 2016 May 24;7:773. doi: 10.3389/fmicb.2016.00773

Table 1.

Treatments used to separate microbial cells from rice rhizoplanes.

Treatment label T1 T2 T3 T4 T5 T6 T7
Treatment procedure Untreated Washed (shaker) Sonication probe1 Sonication probe Sonication bath2 Sonication bath Bleached
Solution – 0.2% PBS-S 0.2% PBS-S 0.2% PBS-S 0.2% PBS-S 0.2% PBS-S 5% NaOCl in PBS
Duration – 15 min 5 × 30 s 2 × 30 s 5 × 30 s 10 × 30 s 2 min
Intensity – 300 rpm 10% power 40% power Low High

1Sonication probe, 3 mm tip (Bandelin Sonopuls HD2200 equipped with MS73). 2Sonication bath with adaptive cavitation technique (Diagenode Bioruptor Plus). Treatments T3–T6 were applied subsequent to washing (T2).