Figure 2.

Twitching motility displayed by A. baumannii clinical isolates. (A) Assays were done using polystyrene Petri dishes by depositing inoculum at the interphase between the bottom of the Petri dish and agar. Plates were incubated for 48 h and stained with crystal violet after discarding the media. The average diameter of the zone of twitching was determined, and the isolates are classified as non-motile (NM, <5 mm), intermediately motile (IM, 5–20 mm), and highly motile (HM, >20 mm) are shown. Representative plates are shown. (B) Bar diagram showing number of isolates (X-axis) displaying NM, IM, and HM phenotypes. Experiments were repeated four or more times.