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. 2016 May 24;4:105. doi: 10.3389/fpubh.2016.00105

Table 1.

Phenotypic properties of the (A) blood isolates and (B) respiratory isolates.

Sample ID Age/sex Clone group# Biofilm* Motility@ (mm) MIC$
 Synergy+
MER SUL COL M + S C + S
(A) Phenotypic properties of the blood isolates
B21029E 25FT EC-II(OEX) 1.61 ± 0.09 10 128 64 0.25 INDa* INDb*
B10883E 4F UC-I 1.95 ± 0.18 >40 64 64 1.0 INDa* INDc*
B29276 0MIV EC-II(OEX) 2.08 ± 0.85 20 16 32 0.5 INDb IND
B25330 0MT EC-III(EX) 2.20 ± 0.71 25 64 64 0.5 INDb* INDc*
B28412 0MT UC-II(OX) 1.01 ± 0.68 <5 32 32 0.5 SYNb* INDc*
B28641E 74MIV UC-III(X) 2.68 ± 0.30 30 64 64 0.5 SYNa* SYNb*
B13833E 75MIV UC-IV(O) 1.03 ± 0.14 35 32 32 0.5 SYNb* INDc*
B1301 54FT,IV UC-I 2.58 ± 0.20 35 128 128 0.5 SYNa INDb,c*
B29819 25M EC-II(OEX) 2.11 ± 0.09 20 128 128 0.5 SYNb* SYNc*
B11439E 45FT EC-II(OEX) 3.11 ± 0.35 10 64 32 0.5 INDb* SYNb*
B27768 50MT EC-II(OEX) 0.73 ± 0.08 10 128 32 0.5 INDa* INDc*
B10631E 13MIV UC-II(OX) 2.06 ± 0.23 10 64 64 0.5 INDb* INDb*
B4609E 13FT,IV EC-II(OEX) 2.15 ± 0.20 25 16 32 0.5 SYNb* INDc*
B27683E 2M UC-V(EO) 3.00 ± 0.48 20 64 32 0.5 SYNa* INDc*
B29467 65M EC-III(EX) 2.43 ± 0.40 >40 64 32 0.5 SYNb* SYNc*
B27919E 34MT,IV EC-II(OEX) 2.45 ± 0.01 10 64 64 0.5 INDa* INDc*
B28414E 74MIV EC-II(OEX) 2.25 ± 0.15 <5 64 64 1.0 SYNb* INDb*
B23356 9MT,IV UC-IV(O) 1.23 ± 0.18 >40 64 32 1.0 INDa INDc*
B8689 41MT,IV UC-IV(O) 0.73 ± 0.02 >40 128 128 0.5 SYNb* INDa*
B17606E 14MT,IV EC-II(OEX) 1.97 ± 0.00 10 64 128 0.5 INDa* INDc*
B8716E 1M EC-II(OEX) 1.61 ± 0.45 10 128 64 0.25 INDa INDc*
B8765 45MT UC-II(OX) 1.24 ± 0.22 <5 256 64 0.5 INDa* INDc*
B11911E/N 45MIV UC-I 2.44 ± 0.78 10 256 64 0.5 IND SYN
B5534 21FT EC-II(OEX) 3.86 ± 0.89 >40 256 64 1.0 SYNb* INDc*
B5208E/N 47M EC-II(OEX) 3.09 ± 0.14 25 128 64 0.5 SYNa* SYNb*
B5586 2FT,IV EC-II(OEX) 2.03 ± 0.20 >40 512 128 1.0 INDa* INDc*
B5868 17MT EC-II(OEX) 1.46 ± 0.41 10 256 64 0.5 SYNb* INDc*
B7370E 24F EC-II(OEX) 1.46 ± 0.10 10 256 64 0.5 SYNa* SYNc*
B8240E 58M EC-II(OEX) 2.10 ± 0.37 15 128 64 0.5 INDa INDc
B9464 24FIV EC-II(OEX) 1.83 ± 0.09 <5 256 64 1.0 SYN IND
(B) Phenotypic properties of the respiratory isolates
SP344 18FIV UC-II(OX) 1.78 ± 0.11 20 64 128 0.25 SYNb* INDc*
SP858 25MT UC-II(OX) 1.37 ± 0.07 <5 128 0.25 0.25 SYNb* INDb*
SP1397E 48MT EC-II(OCX) 3.43 ± 0.11 <5 512 128 0.25 INDb* INDc*
SP1115E 62M EC-II(OCX) 4.72 ± 0.44 <5SM 128 64 0.25 SYNb* INDc*
SP1394 61FT,IV EC-II(OCX) 3.82 ± 0.31 10 64 64 0.25 SYNb* INDc*
SP1182 60MT EC-II(OCX) 4.71 ± 0.25 10 128 64 0.25 INDa* SYNc*
SP1974 57FT,IV EC-II(OCX) 3.67 ± 0.08 <5 256 256 1 INDa* SYNc*
SP1128 35MT EC-III(CX) 3.59 ± 0.16 <5 256 128 0.5 SYNa* INDc*
SP1977 61MT,IV EC-II(OCX) 5.01 ± 0.70 10 256 512 0.5 SYNb SYN
SP1054 39FT,IV EC-II(OCX) 3.90 ± 0.10 <5SM 64 64 1 SYNa INDc*
SP1917E/N 68MT,IV EC-II(OEX) 3.62 ± 0.16 <5 512 256 0.25 SYNa* INDb*
SP1209 49 MT,IV EC-II(OCX) 5.51 ± 0.63 <5SM 64 64 0.5 SYNb* SYNc*
SP1201E 55M EC-II(OCX) 3.72 ± 0.12 10 256 32 0.5 INDa* INDb*
SP932E 52M EC-II(OCX) 3.19 ± 0.12 10 256 64 1 SYNa* INDc*
SP2006E 21MIV UC-IV(O) 1.39 ± 0.09 <5 32 64 8 IND SYN
SP443E 59M UC-II(OX) 1.39 ± 0.00 <5 32 32 0.5 SYNa INDc*
SP1022E 65MIV EC-II(OCX) 2.44 ± 0.35 <5 256 64 1 SYNb INDc*
SP1026E 28MT EC-II(OCX) 3.95 ± 0.33 <5 256 32 1 SYNa* INDc*
SP3179 29MT,IV EC-II(OCX) 3.70 ± 0.17 <5 128 64 1 INDb* INDb,c*
SP2200 40MT,IV UC-II(OX) 2.58 ± 0.30 <5 32 16 1 SYNb* INDc*
SP1950 68MT EC-II(OCX) 3.35 ± 0.58 <5 256 256 0.5 SYNb* INDc*
SP715 17MT UC-II(OX) 3.02 ± 0.63 <5 256 32 0.25 SYNb* SYNc*
SP1909E/N 28F EC-II(OCX) 3.50 ± 0.04 10 512 512 0.5 IND SYN
SP1306E 47FIV UC-II(OX) 1.67 ± 0.03 <5SM 32 64 1 INDb INDc*
SP1766E 40MT EC-II(OCX) 6.78 ± 0.33 >40SM 512 64 0.5 SYNa* SYN
SP1834E 50M EC-II(OCX) 3.08 ± 0.28 <5 256 256 0.5 SYN SYN
SP1840E 75M EC-II(OCX) 6.39 ± 0.47 >40SM 512 128 0.5 SYNa* IND
SP1851E/N 48MT,IV EC-II(OCX) 4.87 ± 0.93 <5 512 256 1 IND IND
SP1843E/N 36F EC-II(OCX) 2.89 ± 0.10 <5 256 128 0.5 SYNb* IND
SP1770 24MT UC-II(OX) 2.14 ± 0.08 <5 128 128 0.5 INDa SYN

#Clonal grouping done based on Turton et al. (39). EC, European clone; UC, unknown clone.

The alleles used for clone determination are O, OmpA; E, CsuE, X, Oxa.

*OD580 values of the crystal violet stain eluted from the biofilm biomass grown on polystyrene tubes.

@Twitching motility scored on Petri plates after staining with crystal violet. Diameter of zone of migration is shown.

$MIC determined by microdilution broth method.

+Determined by time-kill assay.

IND = Indifferent; SYN = Synergy.

MER and M = Meropenem; SUL and S = Sulbactam; COL and C = Colistin.

E: MBL-positive as determined by EDTA plate assay.

N: NDM positive isolates as determined by PCR analysis.

Synergism was determined by time-kill assay only.

Explanation for a/a*, b/b*, and c/c* are: a – meropenem, a* – meropenem positive for bactericidal effect; b – sulbactam, b* – sulbactam positive for bactericidal effect; c – colistin, c* – colistin positive for bactericidal effect.

T, intubated; IV, intravenous lines; SM, swarming motility.