Fig. 2. Effect of sildenafil on cellular ATP content in AGE-treated cells. A : Cells were incubated for indicated time periods in control media with BSA (open circle), in the presence of AGE alone (200 µg/mL, closed circle), or AGE with anti-RAGE antibody (1 µg/mL, solid square). B : Cellular ATP was measured after 48 hr incubation in media containing combinations of AGE, vehicle (Veh), sildenafil citrate (SC, 20 µM), Rp-8-Br-cGMP (RBcGMP, 20 µM). Data were represented as the mean±SEM of 6 experiments. ^*p<0.01 vs. control, ^†p<0.01 vs. Veh, ^‡p<0.01 vs. SC.