Fig. 6. Effect of sildenafil on cytochrome C release in AGE-treated cells. A : Western blot analysis was performed for cytosolic released cytochrome C after incubation for indicated time periods in control media with BSA, in the presence of AGE (200 µg/mL, closed circle), or AGE with sildenafil (SC, 20 µM). B : Western blot analysis was performed after 48 hr incubation in media containing combinations of AGE, vehicle (Veh), sildenafil citrate (SC, 20 µM), Rp-8-Br-cGMP (RBcGMP, 20 µM). Data were represented as the mean±SEM of 6 experiments. ^*p<0.01 vs. control, ^†p<0.01 vs. Veh, ^‡p<0.01 vs. SC.