Fig. 9. Effects of HO-1 related agents on AGE-induced changes in MTT reduction and MPT induction. Cells were incubated in the presence of each 200 µg/mL of control BSA or AGE-BSA (AGE) for 24 hr with or without each 20 µM of sildenafil citrate (SC), cobalt protophorphyrin (CoPP), bilirubin (Bili), and zinc protoporphyrin IX (ZnPP). A : MTT reduction was determined by colorimetric analysis of formazan. B : Confocal microscopic analysis was performed in cells double-stained with TMRM and calcein/AM. Data were represented as the mean±SEM of 4 experiments. *p<0.01 vs. control, †p<0.01 vs. AGE+SC.
