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The recombinant plasmid (pc-NS3) was digested with Xho I and Hind III. The digested plasmid were separated on 1.5% agarose gel and visualized after ethidium bromide staining. Lane 1, undigested pc-NS3; lane2, 1kb DNA ladder marker; lane 3, pc-NS3 digested yields 5.5 kb and 0.9 kb restriction fragments.
