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. 2016 May 24;6:26551. doi: 10.1038/srep26551

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Copyright © 2016, Macmillan Publishers Limited

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Both Cx50WT (A–C) and Cx50R76H (G–I) formed abundant gap junction plaques, as observed at appositional membranes and perinuclear cytoplasmic locations (arrows). Note the enormous aggregation blobs of Cx50R76H in the cytoplasm and plasma membrane (H,I). Images of Cx50P59A transfected cells showed a lack of gap junction plaques; instead, a very low level of expression protein was accumulated in the cytoplasm. Green: GFP; blue: DAPI staining of cell nuclei. The scale bar represents 5 μm.