Figure 5. Bioengineered miR-34a prodrug significantly inhibited the invasion ability of osteosarcoma 143B cells.

Cell images (A; 200× magnification) were acquired with a Olympus IX2-UCB microscope, and invasion capacities were compared for different treatments (B). Cells were treated with 4 nM tRNA/mir-34a or tRNA/MSA, or vehicle for 72 h, subjected to Matrigel invasion assay for 24 h, fixed with 10% formalin, and then stained with 0.1% crystal violet. Values are mean ± SD of six treatments. ***P < 0.001 (one-way ANOVA), as compared with tRNA/MSA or vehicle treatment.