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. 2016 May 24;6:26699. doi: 10.1038/srep26699

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Copyright © 2016, Macmillan Publishers Limited

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(a) Methylation-specific PCR analysis of the promoter CGIs of the indicated lncRNA genes in CRC cell lines, a normal colonic tissue from a healthy individual (24 yo), and normal colonic tissues from CRC patients (1310-N, 74 yo; 1311-N, 75 yo; 1317-N, 79 yo). Bands in the “M” lanes are PCR products obtained with methylation-specific primers. Those in the “U” lanes are products obtained with unmethylation-specific primers. In vitro-methylated DNA (IVD) serves as a positive control. (b) The relationship between DNA methylation and expression of lncRNA genes in CRC cells and a normal colonic tissue. Shown are the results of bisulfite pyrosequencing (black bars) and quantitative RT-PCR (white bar) analysis of the four selected lncRNA genes. RT-PCR results were normalized to the internal RPL19 expression.