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. 2016 May 24;6:26699. doi: 10.1038/srep26699

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Copyright © 2016, Macmillan Publishers Limited

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(a) Levels of DNA methylation in the promoter CGI of ZNF582-AS1 in the indicated tumors (C) and corresponding normal tissues (N) analyzed using TCGA data sets. STAD: stomach adenocarcinoma, COAD: colon adenocarcinoma, READ: rectum adenocarcinoma, ESCA: esophageal carcinoma, HNSC: head and neck squamous cell carcinoma, CESC: cervical and endocervical cancer, PAAD: pancreatic adenocarcinoma, BRCA: breast invasive carcinoma, LUAD: lung adenocarcinoma, LUNG: lung cancer, LUSC: lung squamous cell carcinoma, LIHC: liver hepatocellular carcinoma, KIRC: kidney clear cell carcinoma, KICH: kidney chromophobe, KIRP: kidney papillary cell carcinoma, SKCM: skin cutaneous melanoma, LGG: brain lower grade glioma, LAML: acute myeloid leukemia, DLBC: diffuse large B-cell lymphoma, SARC: sarcoma. (b) Levels of ZNF582-AS1 methylation in gastric cancer cell lines and normal gastric mucosa analyzed using bisulfite pyrosequencing. (c) Quantitative RT-PCR analysis of ZNF582-AS1 and ZNF582 in a series of gastric cancer cell lines and normal stomach tissues. The results are normalized to internal RPL19 expression.