Table 1. Relative amounts (%) of maize seed proteins.
| 20 DAF |
60 DAF |
|||
|---|---|---|---|---|
| CTRL | MIC | CTRL | MIC | |
| Albumin | 34.7 ± 5.3 aA | 31.1 ± 6.2 bA | 15.1 ± 1.7 aB | 10.3 ± 2.4 bB |
| Globulin | 8.1 ± 4.4 aA | 5.3 ± 2.6 aA | 9.9 ± 2.2 aA | 16.8 ± 2.2 bB |
| Prolamins | 32.1 ± 6.1 aA | 41.9 ± 5.7 aA | 44.6 ± 3.1 aB | 47.4 ± 2.6 aA |
| Glutelins | 20.0 ± 5.4 aA | 21.6 ± 5.2 aA | 27.9 ± 3.4 aA | 25.2 ± 3.9 aA |
Seed proteins were classified in groups according to their solubility in a series of solvents including albumin (soluble in water), globulins (soluble in dilute aqueous salt solution), prolamins (soluble in alcohol solution) and glutelins (soluble in dilute alkali). Data are expressed as means ± standard error (Three biological samples were analysed twice in duplicate). ANOVA followed by Fisher’s probable least-squares difference test used a cut-off significance at p = 0.05. Different letters indicate significantly different values based on one-way ANOVA (P < 0.05). Small letters indicate comparison between treatments (CTRL vs MIC) at the same time (20 or 60 DAF); capital letters indicate comparison between different times (20 DAF vs 60 DAF) in the same treatment (CTRL or MIC).