. 2016 May 24;6:26439. doi: 10.1038/srep26439

Table 2. Information regarding modulated proteins spot number, number of identified peptides, sequence coverage, optical density variation using colour code, ANOVA P-value, protein name and Blast results when present, theoretical molecular weight and pI (experimental pI data were not reported because the isoelectrofocusing was performed on non-linear IPG strips and image analysis software was not able to precisely calculate the pI), accession number and reference organism, biological process in which the identified protein was involved.

Spot	N. Peptides	Seq. Coverage	P-Value	Protein name/Blast result	Mr (kDa)/pI Theor	AC number/reference organism	Biological process
114	8	47%	<0.0001	Unknown/Adenine phosphoribosyl transferase	19336/5.14	gi\|194701624/Zea mays	Adenine salvage
381	11	18%	0.0357	Acetolactate synthase 1	68887/6.69	gi\|75102649/Zea mays	Amino-acid biosynthesis
219	7	22%	<0.0001	ATP synthase beta chain	45679/4.92	gi\|149798689/Eriosorus cheilanthoides	ATP synthesis coupled proton transport
388	3	8%	0.0344	IAA-glu synthetase	49679/5.75	gi\|162460991/Zea mays	Auxin conjugation
24	5	7%	<0.0001	Putative aconitate hydratase	98021/5.67	gi\|75225211/Oryza sativa	Carbohydrate metabolism
164	9	22%	<0.0001	Phosphoglucomutase 2	63002/5.47	gi\|162459678/Zea mays	Carbohydrate metabolism
274	12	34%	0.0135	Sorbitol dehydrogenase	39063/6.27	gi\|77378040/Zea mays	Carbohydrate metabolism
380	13	37%	0.0018	Fructokinase 2	35459/5.34	gi\|162460525/Zea mays	Carbohydrate metabolism
54	11	22%	<0.0001	Phosphoglucomutase 1	63058/5.46	gi\|162463106/Zea mays	Carbohydrate metabolism/glucose metabolism
67	17	36%	<0.0001	Phosphoglucomutase 2	63002/5.47	gi\|162459678/Zea mays	Carbohydrate metabolism/glucose metabolism
245	3	8%	0.0272	Phosphoglucomutase 2	63002/5.47	gi\|162459678/Zea mays	Carbohydrate metabolism/glucose metabolism
112	12	53%	0.0002	Unknown/Aldolase 1	38566/7.52	gi\|194690156/Zea mays	Carbohydrate metabolism/Glycolysis
136	16	41%	0.0002	Phosphoglycerate mutase	60592/5.29	gi\|551288/Zea mays	Carbohydrate metabolism/Glycolysis
145	20	62%	<0.0001	3-phosphoglycerate kinase	42413/5.65	gi\|194707626/Zea mays	Carbohydrate metabolism/Glycolysis
163	14	63%	0.0002	Glyceraldehyde-3-phosphate dehydrogenase	24930/8.44	gi\|293887/Zea mays	Carbohydrate metabolism/Glycolysis
196	18	61%	0.0004	Glyceroldehyde-3-phosphate dehydrogenase	36428/6.61	gi\|162458671/Zea mays	Carbohydrate metabolism/Glycolysis
205	17	64%	0.0045	Glyceroldehyde-3-phosphate dehydrogenase	36519/6.41	gi\|162461501/Zea mays	Carbohydrate metabolism/Glycolysis
249	4	7%	<0.0001	Phosphoglycerate mutase	60592/5.29	gi\|551288/Zea mays	Carbohydrate metabolism/Glycolysis
251	10	49%	0.0003	Glyceroldehyde-3-phosphate dehydrogenase	36428/6.61	gi\|162458671/Zea mays	Carbohydrate metabolism/Glycolysis
278	10	35%	0.0070	Enolase1	48033/5.20	gi\|162458207/Zea mays	Carbohydrate metabolism/Glycolysis
305	15	67%	0.0002	Glyceraldehyde-3-phosphate dehydrogenase	24930/8.44	gi\|293887/Zea mays	Carbohydrate metabolism/Glycolysis
309	4	9%	0.0112	Unknown/Phosphofructokinase	60980/5.96	gi\|194700662/Zea mays	Carbohydrate metabolism/Glycolysis
311	17	51%	0.0013	Unknown/Phosphoglycerate kinase	42413/5.65	gi\|194707626/Zea mays	Carbohydrate metabolism/Glycolysis
321	17	48%	0.0079	Enolase1	48033/5.20	gi\|162458207/Zea mays	Carbohydrate metabolism/Glycolysis
326	2	8%	<0.0001	Enolase1	48033/5.20	gi\|162458207/Zea mays	Carbohydrate metabolism/Glycolysis
368	10	31%	0.0100	Enolase2	48132/5.70	gi\|162460735/Zea mays	Carbohydrate metabolism/Glycolysis
36	7	42%	<0.0001	Prohibitin3	30580/7.00	gi\|162462359/Zea mays	Cell growth
45	4	22%	<0.0001	Prohibitin 2	30702/6.55	gi\|162462211/Zea mays	Cell growth
358	26	55%	0.0018	Protein disulfide isomerase	56838/5.01	gi\|162461063/Zea mays	Cell redox homeostasis
29	4	40%	<0.0001	Actin depolymerizing factor	15890/5.46	gi\|162459533/Zea mays	Cytoskeleton
170	5	40%	<0.0001	Actin depolymerizing factor	15890/5.46	gi\|162459533/Zea mays	Cytoskeleton
252	11	51%	0.0003	Actin	41699/5.24	gi\|53759189/Saccharum officinarum	Cytoskeleton
340	7	21%	0.0033	Hypothetical protein LOC100191561/Actin	41699/5.24	gi\|212274479/Zea mays	Cytoskeleton
398	7	40%	0.0409	Hypothetical protein LOC100193683/Proteasome subunit alpha type 2	25848/5.53	gi\|212720956/Zea mays	Defense response to bacterium
382	2	8%	0.0145	Unknown/Ankyrin repeat domain-containing protein 2	36227/4.50	gi\|194707992/Zea mays	Defense response to bacterium, incompatible interaction
61	15	91%	<0.0001	Late embryogenesis abundant protein Lea14-A	16078/8.05	gi\|195658529/Zea mays	Defense response to dessiccation
104	3	14%	0.0007	Unknown/Dessication-related protein	34010/4.82	gi\|194708240/Zea mays	Defense response to dessiccation
1	3	16%	<0.0001	Late embryogenesis abundant protein, group 3	18588/7.85	gi\|195605580/Zea mays	Embryo development ending in seed dormancy
363	9	44%	0.009	Unknown/APx1 - Cytosolic Ascorbate Peroxidase	27368/5.65	gi\|195654277/Zea mays	Embryo development ending in seed dormancy
385	10	22%	0.0314	Unknown/Vacuolar ATP synthase catalytic subunit A	68376/5.30	gi\|195658441/Zea mays	Embryo development ending in seed dormancy
387	10	48%	0.0012	Unknown/APx1 - Cytosolic Ascorbate Peroxidase	27368/5.65	gi\|195654277/Zea mays	Embryo development ending in seed dormancy
403	7	36%	0.0189	Unknown/APx2 - Cytosolic Ascorbate Peroxidase	27211/5.28	gi\|194707280/Zea mays	Embryo development ending in seed dormancy
328	4	8%	0.0273	Unknown/2-isopropylmalate synthase B	67138/7.02	gi\|195604800/Zea mays	Glucosinolate biosynthesis process
339	17	30%	0.0463	Unknown/2-isopropylmalate synthase B	67138/7.02	gi\|195604800/Zea mays	Glucosinolate biosynthesis process
185	2	3%	0.0003	Putative aconitate hydratase	98021/5.67	gi\|75225211/Oryza sativa	Glyoxylate and dicarboxylate metabolism
186	3	6%	0.0009	Putative aconitate hydratase	98021/5.67	gi\|75225211/Oryza sativa	Glyoxylate and dicarboxylate metabolism
147	5	16%	<0.0001	Catalase isozyme 1	56841/7.40	gi\|115679/Zea mays	Hydrogen peroxide
399	3	7%	0.0451	Non-photosynthetic NADP-malic enzyme	70622/6.46	gi\|37147841/Zea mays	Malate metabolic process
9	2	17%	<0.0001	Unknown/Splicing factor	19898/11.53	gi\|194695412/Zea mays	Nucleic acid binding
266	2	10%	0.0093	Glycine-rich RNA binding protein	15908/5.22	gi\|20257707/Zea mays	Nucleic acid binding
282	2	9%	0.0006	Unknown/Plasminogen activator inhibitor 1 RNA-binding protein	40439/5.72	gi\|194701098/Zea mays	Nucleic acid binding
111	3	7%	0.0001	Nucleoside diphosphate kinase 1	16835/6.30	gi\|50096951/Oryza sativa	Nucleotide metabolism
319	4	19%	0.0144	Nucleoside diphosphate kinase 1	16835/6.30	gi\|50096951/Oryza sativa	Nucleotide metabolism
69	16	54%	<0.0001	Unknown/Glucose and ribitol dehydrogenase homolog	32924/5.78	gi\|194699516/Zea mays	Oxidation-reduction process
334	6	29%	0.0023	Carbonyl reductase 1	32662/6.16	gi\|195650645/Zea mays	Oxidation-reduction process
335	3	20%	<0.0001	Unknown/Peroxiredoxin	17312/4.85	gi\|194698866/Zea mays	Oxidation-reduction process
43	17	27%	<0.0001	C4-specific pyruvate orthophosphate dikinase	102343/5.50	gi\|31322754/Miscanthus x giganteus	Photosynthesis
52	34	51%	<0.0001	Chain A, Pyruvate Phosphate Dikinase	95132/5.27	gi\|62738111/Zea mays	Photosynthesis
38	28	43%	<0.0001	Pyruvate orthophosphate dikinase	102444/5.71	gi\|168586/Zea mays	Photosynthesis
53	12	22%	<0.0001	Pyruvate orthophosphate dikinase	102444/5.71	gi\|168586/Zea mays	Photosynthesis
74	13	18%	<0.0001	Pyruvate orthophosphate dikinase	102471/5.52	gi\|6274486/Saccharum officinarum	Photosynthesis
96	13	19%	0.0002	Pyruvate orthophosphate dikinase	102471/5.52	gi\|6274486/Saccharum officinarum	Photosynthesis
107	17	21%	<0.0001	Pyruvate orthophosphate dikinase	102471/5.52	gi\|6274486/Saccharum officinarum	Photosynthesis
207	2	10%	0.0047	QM protein	24903/10.27	gi\|162458844/Zea mays	Photosynthesis
276	5	10%	0.0014	Pyruvate, orthophosphate dikinase	102444/5.71	gi\|168586/Zea mays	Photosynthesis
108	14	24%	0.0006	Os02g0519900/Elongation factor 2	93961/5.85	gi\|115446385/Oryza sativa	Protein biosynthesis
139	2	10%	<0.0001	Translation initiation factor 5A	17486/5.61	gi\|162458009/Zea mays	Protein biosynthesis
174	13	35%	0.0003	Unknown/Eukariotic translation initiation factor 3 subunit 7	64846/5.51	gi\|194704818/Zea mays	Protein biosynthesis
178	12	23%	<0.0001	Os02g0519900/Elongation factor 2	93961/5.85	gi\|115446385/Oryza sativa	Protein biosynthesis
360	12	38%	0.0041	Translational initiation factor eIF-4A	46952/5.37	gi\|162458395/Zea mays	Protein biosynthesis
286	5	27%	0.0055	Unknown/Proteasome subunit alpha type 5	25961/4.76	gi\|195635461/Zea mays	Protein catabolic process
66	5	39%	<0.0001	Unknown/Putative chaperonin 21 precursor	25739/8.49	gi\|194688414/Zea mays	Protein folding
265	10	27%	0.0005	Os02g0102900 /RuBisCO large subunit-binding protein	63759/5.77	gi\|115443643/Oryza sativa	Protein folding
284	16	33%	0.0145	Unknown/T-complex protein 1 subunit alpha	59158/5.78	gi\|195636596/Zea mays	Protein folding
287	11	22%	0.0211	Os06g0114000/Chaperonin 60 Beta	64046/5.60	gi\|115466004/Oryza sativa	Protein folding
318	9	48%	0.0003	Peptidyl-prolyl cis-trans isomerase	18337/8.91	gi\|118104/Zea mays	Protein folding
86	13	22%	0.0029	OSJNBa0039C07.4/ATP dependent Clp protease ATP-binding subunit	98436/5.79	gi\|38347158/Oryza sativa	Protein metabolic process
148	7	13%	<0.0001	Os12g0230100/ATP dependent Clp protease	101954/6.62	gi\|115487910/Oryza sativa	Protein metabolic process
338	3	34%	0.0100	Unknown/NADH ubiquinone oxidoreductase B22-like subunit	13346/8.01	gi\|195605254/Zea mays	Respiratory chain
214	4	15%	<0.0001	Glyoxalase I	32336/5.59	gi\|162461576/Zea mays	Response to salt stress
292	13	25%	0.0416	Unknown/Ketol-acid reductoisomerase	62963/6.31	gi\|194693902/Zea mays	Response to salt stress
337	11	27%	0.0084	Alanine aminotransferase 2	53000/6.23	gi\|195625602/Zea mays	Response to salt stress
357	7	18%	0.0293	Adenosine kinase	36009/5.23	gi\|4582787/Zea mays	Response to salt stress
12	6	41%	<0.0001	Unknown/Transcription factor homolog	17757/6.62	gi\|194695608/Zea mays	Response to salt stress
134	13	19%	<0.0001	Putative aconitate hydratase 1	106913/6.63	gi\|92429669/Sorghum bicolor	Response to salt stress
189	3	27%	0.0034	Unknown/Mitochondrial F0 ATP synthase D chain	19915/5.19	gi\|194701816/Zea mays	Response to salt stress
192	10	20%	0.0014	Methionine synthase protein	83736/5.93	gi\|18483235/Sorghum bicolor	Response to salt stress
273	7	44%	0.0003	Unknown/Superoxide dismutase 3	25571/7.11	gi\|194689068/Zea mays	Response to salt stress
395	8	29%	0.019	Hypothetical protein LOC100191638/Salt tolerance protein	35252/4.92	gi\|212274681/Zea mays	Response to salt stress
15	3	6%	<0.0001	Vicilin-like embryo storage protein	66122/6.23	gi\|22284/Zea mays	Seed storage
21	4	19%	0.0004	Zein-alpha 19D1 precursor	26616/9.21	gi\|162458484/Zea mays	Seed storage
26	3	6%	<0.0001	Vicilin-like embryo storage protein	66122/6.23	gi\|22284/Zea mays	Seed storage
44	9	20%	<0.0001	Vicilin-like embryo storage protein	66122/6.23	gi\|22284/Zea mays	Seed storage
51	3	6%	<0.0001	Vicilin-like embryo storage protein	66122/6.23	gi\|22284/Zea mays	Seed storage
188	4	14%	0.0031	Vicilin-like embryo storage protein	66122/6.23	gi\|22284/Zea mays	Seed storage
242	7	19%	0.0010	Vicilin-like embryo storage protein	66122/6.23	gi\|22284/Zea mays	Seed storage
261	1	10%	0.0229	Zein protein precursor	19448/8.05	gi\|168664/Zea mays	Seed storage
354	4	6%	0.0187	Vicilin-like embryo storage protein	66122/6.23	gi\|22284/Zea mays	Seed storage
64	1	23%	0.0003	z1B alpha zein protein	16047/8.00	gi\|157780962/Zea mays	Seed storage (nutrient reservoir activity)
179	10	34%	0.0135	Legumin 1	52798/6.20	gi\|162460908/Zea mays	Seed storage (nutrient reservoir activity)
50	11	51%	<0.0001	Unknown/Chitinase	29193/8.44	gi\|194702870/Zea mays	Somatic embryogenesis/fruit development
210	11	22%	0.0232	Granule-bound starch synthase precursor	66567/6.59	gi\|33321047/Zea mays	Starch metabolic process
342	10	22%	0.0036	Granule-bound starch synthase precursor	66567/6.59	gi\|33321047/Zea mays	Starch metabolic process
83	10	38%	0.0002	Unknown/Stress responsive protein	38371/6.30	gi\|194707628/Zea mays	Stress response
4	6	30%	<0.0001	22.0 kDa class IV heat shock protein precursor	22872/6.01	gi\|195644560/Zea mays	Stress response
13	3	26%	<0.0001	Heat shock protein 17.2	17152/5.54	gi\|162459222/Zea mays	Stress response
49	4	16%	0.0018	Activator of 90 kDa heat shock protein ATPase	38577/5.33	gi\|195651993/Zea mays	Stress response
55	10	21%	<0.0001	Heat shock 70 kDa protein	72704/5.62	gi\|195649437/Zea mays	Stress response
59	14	54%	<0.0001	Unknown/Stress responsive protein	38371/6.30	gi\|194707628/Zea mays	Stress response
87	2	3%	0.0059	Putative heat shock protein	82531/5.43	gi\|37718900/Oryza sativa	Stress response
149	15	26%	0.0027	Heat-shock protein 101	101069/5.85	gi\|162458166/Zea mays	Stress response
154	10	21%	0.0036	Unknown/NADH ubiquinone oxidoreductase 75 kDa subunit	80628/6.10	gi\|194688928/Zea mays	Stress response
172	8	31%	<0.0001	Lactoylglutathione lyase	35140/6.62	gi\|195639070/Zea mays	Stress response
197	8	42%	<0.0001	Unknown/Stress responsive protein	37857/6.70	gi\|194703432/Zea mays	Stress response
213	4	42%	0.0002	Hypothetical protein Z477F24.14/Lactoylglutathione lyase	15597/4.94	gi\|48374986/Zea mays	Stress response
272	6	41%	0.0012	Hypothetical protein LOC100191552/Heat shock protein 17.9	17869/6.86	gi\|212276212/Zea mays	Stress response
312	11	41%	<0.0001	Unknown/Stress responsive protein	37857/6.70	gi\|194703432/Zea mays	Stress response
356	18	29%	0.0149	Unknown/Stromal 70 kDa heat shock-related protein	74625/5.08	gi\|195657157/Zea mays	Stress response
376	15	21%	0.0134	Unknown/Heat shock 70 kDa protein	72704/5.62	gi\|195649437/Zea mays	Stress response
352	7	19%	<0.0001	Unknown/Putative aminotransferase	49566/6.55	gi\|195634861/Zea mays	Systemic acquired resistance
336	4	32%	0.0030	Unknown/40S ribosomal protein S7	22198/9.76	gi\|195605060/Zea mays	Translation
268	6	32%	<0.0001	Malate dehydrogenase 5	35567/5.77	gi\|162464321/Zea mays	Tricarboxylic acid cycle
394	5	12%	0.0150	Succinate dehydrogenase flavoprotein subunit	67941/6.08	gi\|195647178/Zea mays	Tricarboxylic acid cycle
5	3	8%	<0.0001	Fasciclin-like arabinogalactan protein 8 precursor	44699/6.56	gi\|195607426/Zea mays	Unknown
78	8	49%	0.0013	Unknown/Carbonyl reductase	20867/6.84	gi\|194701990/Zea mays	Unknown
206	5	53%	0.0009	Chain A, Bifunctional Hageman Factor AMYLASE INHIBITOR FROM MAIZE	13570/6.51	gi\|157830250/Zea mays	Unknown
329	2	12%	0.0066	Unknown/DREPP4 protein	22630/4.89	gi\|194690236/Zea mays	Unknown
343	5	16%	0.0098	Unknown/Seed protein	26645/7.11	gi\|195626982/Zea mays	Unknown
362	3	13%	<0.0001	Unknown/Malonyl-CoA:ACP transacylase 1-3	38593/5.64	gi\|195638470/Zea mays	Unknown
392	11	24%	0.0400	Unknown/UDP-glucose pyrophosphorylase	52056/5.30	gi\|194688950/Zea mays	Unknown
17	7	38%	0.0013	Rab28	27693/4.90	gi\|22460/Zea mays	Unknown
6			0.0141	ND
11			<0.0001	ND
40			<0.0001	ND
175			0.0003	ND
200			0.0045	ND
203			<0.0001	ND
257			0.0001	ND
262			<0.0001	ND
267			0.0071	ND
389			0.0034	ND