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. 2016 Apr 13;5(6):404–414. doi: 10.1016/j.molmet.2016.04.003

Figure 2.

Figure 2

Glucose preferentially increases C16:0-containing, di-saturated DAG species in primary islets (A–C) and MIN6 cells (D–E). Cellular lipids were extracted and then subjected to MS, as per Methods. Results are presented as mass normalized to total phospholipid content (A–C) or protein content (D–E) following stimulation with 2 mM (white bars) or 20 mM glucose (grey bars) for 1 h. DAG species are grouped as containing side chains that are di-saturated (A,D), mixed (B,E) or containing C20:4 (C,F). *p < 0.05, **p < 0.01, Student's unpaired t-test assuming similar scatter in these populations, and corrected for multiple comparisons (Holm Sidak). n = 7–9 experiments (A) or n = 6–7 experiments (B).